Author: Pipette Supplies Team

mLINE Calibration Instructions:

ATTENTION: Performing a calibration adjustment on any pipette should always be the last option, never the first. Performing a calibration adjustment first may mask underlying pipette performance issues that could affect both the short- and long-term health of your pipette. This could lead to costly repairs and part(s) replacement, and possibly the need to replace the pipette itself.  Adjusting the pipette calibration should only occur after the pipette sealing system, (i.e.: piston, shaft, o-ring, lubricant, pipette tips) and other critical components are inspected to ensure good working condition. If not replace part(s) as needed first. If you are unsure if the parts are in good condition or which parts should be replaced, please contact Pipettesupplies.com.

Printable Calibration Log
Printable Calibration Stickers (for use with Avery 5960 labels)

Recommended mLine Pipette Calibration Adjustment Tools and Needed Equipment:

• mLine Calibration Tool (Pipette Supplies Part Number SA 726203)
• Pipette Tips (for checking the volume delivered after calibration adjustment is made)
• Di Water non-aerated (for verifying the accuracy/precision after a calibration adjustment)
• 4-5 Place Balance (to gravimetrically confirm the desired volume aspirated and delivered is accurate and precise.)

Calibration Adjustment Process:

Step 1: Insert part B of the calibration tool into the calibration cap cover (SA 726006) that is located on the backside of the pipette body and gently pry off and remove the calibration cap. Lay aside in a safe location for re-installation later on. The calibration screw is now visible.

Step 2: (NOTE) Notice that the calibration screw will have a + and – symbol etched on it to indicate which direction the calibration adjustment tool should be turned to either increase (+) or decrease (-) the desired volume aspirated and dispensed by the pipette.

Step 3: (NOTE) If the current actual volume is lower or higher than desired then insert calibration tool:

Lower than desired: Slowly rotate the calibration adjustment tool no more than 1/8 of a turn in the counter–clockwise direction. This will increase the volume of liquid aspirated.

Higher than desired: Slowly rotate the calibration adjustment tool no more than 1/8 of a turn in the clockwise direction. This will decrease the volume of liquid aspirated.

Important: It is strongly recommended that any calibration adjustments occur at the 10% volume. This helps to ensure accuracy and precision is maintained throughout the pipette volume range (i.e., 20uL-200uL, 100uL -1000uL, etc.). Failure to do this could skew or affect the volume aspirated and dispensed at different tested volume points.

After Calibration Adjustment Process:

• Remove the calibration adjustment tool from the pipette calibration screw
• Install a pipette tip onto the pipette shaft
• Pre-rinse the tip 2-3 times with Di-water
• Aspirate and dispense the di water into a reservoir on a 4-5 place balance to confirm the new volume that is now being delivered
• Repeat the calibration adjustment process if the new volume delivered is still too low or too high

(NOTE) If the pipette is not delivering the desired volume after performing calibration adjustments for 2-3 minutes, lay the pipette aside and allow it to equilibrate back to ambient lab conditions for 60 minutes. After this time period lapses, re-check the pipette calibration (i.e., due to possible hand warming effects).

Step 4: After the desired volume is verified on a 4-5 place balance, re-install the calibration cap. The mLINE pipette is now ready for use.

Replace a mLINE Tip Ejector:

Step 1 Disassembly: 

Gently pry and lift off the colored plunger button cap from the mLINE pipette and lay aside for reassembly later on. Shop all colors here.

Step 2:  

Gently push up on the tip ejector button with your thumb to loosen/detach the black or blue colored body collar from the pipette. Lift and lay aside for reassembly later.

Step 3:

Continue to gently slide and remove the tip ejector mechanism from the pipette body until it is fully removed.

Step 3A:

With the tip ejector removed, you should have these three parts laid aside:

• New mLINE replacement Tip Ejector (gray or blue colored): SA 726005
• Body Collar: SA 726002
• Plunger Button Cap: Shop all colors here

Note: The tip ejector collars and tip ejector may come in a blue or gray color. Other than color, they are both the same and interchangeable

Step 4 Reassembly:

Gently line up the tip ejector posts with the body slots. Once aligned, slowly insert and slide the new tip ejector assembly fully into place.

Step 5 Reassembly:

Re-attach the blue or black colored collar assembly. Gently push it down until it seats firmly in place.

Step 6 Reassembly:

Re-install the plunger button onto the pipette plunger rod.

Step 7 Reassembly:

Test out the newly replaced mLINE tip ejector by installing and ejecting pipette tips.

Disclaimer: Any action you take using the information on this website is strictly at your own risk. The information herein does not constitute professional advice and is general in nature. We make no warranty that this information will meet your requirements, be safe, accurate, or error-free. Pipette Supplies, Inc. is not responsible for any errors or omissions, any results obtained from the use of this information, or any loss or damage arising out of the use of this information. This site is for educational purposes only.
Fair Use: Copyright Disclaimer under section 107 of the Copyright Act of 1976, allows for “fair use” for purposes such as comments, criticism, teaching, scholarship, news reporting, and research.
Fair use is permitted by copyright statute that might otherwise be infringing.

Sartorius mLINE 5000uL Piston Cleaning & Preventive Maintenance

Recommended Tools and Parts:

• Foam Swabs (cotton swabs not recommended)
• Sartorius Lubricant (SA LH-731141)
• Lint-free towels (kim wipes)

Note: As Preventive Maintenance (PM) is performed, there may be a need to replace one or more of the following parts based on pipette part condition and wear and tear.

• mLINE Piston
• O-ring
• Shaft
• Lubricant
• Shaft, O-Ring, & Piston Assembly (SA 726097). Only sold as a kitted item not individually as shown.

Step 1:  

Loosen the outer shaft sleeve by carefully unscrewing it. Once unscrewed, remove and lay aside for reassembly later.

Step 2:

Loosen the black colored shaft assembly from the body by carefully unscrewing it. Once fully unscrewed, lay aside for preventive maintenance

Note: When the black shaft assembly is removed, you may see visible lubricant on the piston as well as inside the large orifice open of the shaft. This is okay and will be cleaned during PM.

Step 3:

With the piston now exposed, use a clean kim wipe to gently wipe and remove any lubricant, chemical splash, or debris from the piston as well as inside the large portion of the shaft opening.

Note: Repeated wiping with the same kim wipe is not recommended. Please wipe only once and disregard to avoid scratching the piston, piston o-ring, and inside of the shaft.

Step 4:

With the shaft now exposed, use a clean kim wipe to gently wipe and remove any lubricant from the piston as well as inside the large portion of the shaft opening.

Note: Be sure the piston and shaft do not have any visible amounts of lubricant after cleaning.

Step 5:

With a clean foam swap, apply a thin amount of the Sartorius pn: SA LH-731141 onto to it. Gently coat the piston o-ring with a thin layer of lubricant around the entire circumference of the piston o-ring.

DO NOT over apply with a thick coat of lubricant. Just enough for it to be seen visibly on the piston o-ring.

Step 6 Reassembly:

Reattach and carefully screw the black shaft onto the mLINE piston assy.

Step 7 Reassembly:

Reattach the outer shaft sleeve to the piston by carefully screwing it onto the body.

Helpful Tip: Once the pipette is fully reassembled, set the pipette volume to 5000uL and place a 5000uL tip onto the shaft.

• Fully aspirate 5000uL Di water into the tip
• Slowly withdrawal the tip from the di water reservoir
• With the water still in the tip, count to 10. The purpose is to see if any visible leaks will occur before proceeding to the next step.
  • If a leak is detected, please disassemble, remove lubricant, and reapply. If leak still occurs then it may be possible that the piston, o-ring, and or shaft may need to be replaced.

Step 8:

After a successful liquid leak test, allow the pipette to equilibrate for at least 60 minutes to ambient conditions after preventive maintenance (PM) is complete.

Step 9:

Once the equilibration period has ended, verify pipette accuracy and precision on a 4-5 place balance. If pipette meets acceptable accuracy and precision requirements then use freely. If it does not, please contact us at Pipettesupplies.com for additional support.

Printable Calibration Log
Printable Calibration Stickers (for use with Avery 5960 labels)

Disclaimer: Any action you take using the information on this website is strictly at your own risk. The information herein does not constitute professional advice and is general in nature. We make no warranty that this information will meet your requirements, be safe, accurate, or error-free. Pipette Supplies, Inc. is not responsible for any errors or omissions, any results obtained from the use of this information, or any loss or damage arising out of the use of this information. This site is for educational purposes only.
Fair Use: Copyright Disclaimer under section 107 of the Copyright Act of 1976, allows for “fair use” for purposes such as comments, criticism, teaching, scholarship, news reporting, and research.
Fair use is permitted by copyright statute that might otherwise be infringing.

How to replace a mLINE volume display window:

Required Tools and Parts:

• Sartorius Calibration Tool – SA 726203
• Small flat head screw driver
• mLINE Volume Display Windows – Shop all volumes here
• Volume Lock Button (Counter Release Nut) – SA LH-726221

Note: Only replace the mLINE volume display window if it is cracked or if the tabs that hold it in place have broken off.

Step 1:  

With Part B of the Sartorius calibration tool or a small bladed flat head screwdriver, gently pry/lift up a corner of the volume display window and remove.

Note: Be careful as the volume lock button may fall off from the window display during removal. Lay side for reassembly later on.

Step 2:

If the volume lock button did not come off during removal of the volume window, carefully remove and lay aside as shown.

• The volume lock button may be gray or blue colored. Other than color, they are interchangeable.

Step 3 Reassembly:

Ensure that the three volume display window tabs are not cracked or broken.

Step 3A:

Align the volume lock button tabs with the volume display window and insert into place.

Step 4:

Once the volume lock cap is inserted into the volume display window:

• Align the volume display three (3) window tabs with the pipette body.

• Carefully seat/secure the volume display window in place. Be extra careful to not damage or break the volume display window tabs during installation. Alignment is critical to avoid breakage.

Step 5:

Once reassembled, test the volume adjustment by pressing the volume lock button and slowly adjusting the pipette volume. If the volume does not turn smoothly, recheck your work. Confirm that the volume lock button is properly seated.

Disclaimer: Any action you take using the information on this website is strictly at your own risk. The information herein does not constitute professional advice and is general in nature. We make no warranty that this information will meet your requirements, be safe, accurate, or error-free. Pipette Supplies, Inc. is not responsible for any errors or omissions, any results obtained from the use of this information, or any loss or damage arising out of the use of this information. This site is for educational purposes only.
Fair Use: Copyright Disclaimer under section 107 of the Copyright Act of 1976, allows for “fair use” for purposes such as comments, criticism, teaching, scholarship, news reporting, and research.
Fair use is permitted by copyright statute that might otherwise be infringing.